CRISPR-Cas9 Induced Knockout of BEL5 in Tetraploid Potato: Optimized Methodology via Repeated de novo Regeneration and Impact on Tuberization
This study establishes an optimized CRISPR-Cas9 methodology involving repeated de novo regeneration to achieve complete knockout of the BEL5 gene in tetraploid potato, revealing that while BEL5 regulates the timing of tuberization onset, it is dispensable for overall tuber yield.